Trypanocidal effect of Diminazene aceturate by intranasal administration. Comparison among formulations

Authors

  • Fuchs AG Instituto Nacional de Parasitología, ANLIS “Carlos G. Malbrán”, C1063 Buenos Aires, Argentina.
  • Soraires Santacruz MC Cátedra de Química Medicinal, Departamento de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, C1027AAP Buenos Aires, Argentina. Instituto de Química y Metabolismo del Fármaco (IQUIMEFA), Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, C1027AAP Buenos Aires, Argentina.
  • Fusco OA Instituto Nacional de Parasitología, ANLIS “Carlos G. Malbrán”, C1063 Buenos Aires, Argentina.
  • Hoffer AM Instituto Nacional de Parasitología, ANLIS “Carlos G. Malbrán”, C1063 Buenos Aires, Argentina.
  • Zhang CX Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, P.R. China.
  • Zhang F Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, P.R. China.
  • Esteva MI Instituto Nacional de Parasitología, ANLIS “Carlos G. Malbrán”, C1063 Buenos Aires, Argentina.
  • Rial MS Instituto Nacional de Parasitología, ANLIS “Carlos G. Malbrán”, C1063 Buenos Aires, Argentina.
  • Fichera LE Instituto Nacional de Parasitología, ANLIS “Carlos G. Malbrán”, C1063 Buenos Aires, Argentina.
  • Cid NG Instituto de Nanobiotecnología (NANOBIOTEC), Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, C1027AAP Buenos Aires, Argentina.
  • Glisoni RJ Instituto de Nanobiotecnología (NANOBIOTEC), Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, C1027AAP Buenos Aires, Argentina.
  • Lai DH Center for Parasitic Organisms, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, P.R. China.
  • Bontempi EJ Instituto Nacional de Parasitología. ANLIS Malbrán. Ministerio de Salud

Keywords:

Berenil, Trypanosomiasis, Intranasal, Diminazene aceturate, Chitosan, Oil

Abstract

Background: The standard treatment for nagana and surra, vector–born parasite diseases, is a single intramuscular (i.m.) dose of diminazene aceturate (Berenil), an aromatic diamidine. Due to discontinuation of public provision of veterinary services or to lack of access to remote areas, low income farmers inject livestock and other domestic animals by themselves. We tested a not explored administration route for drugs against parasitological infections: the intranasal one.  Berenil alone dissolved in water did not reach an effective drug concentration.

Methods: Mice were infected with Trypanosoma brucei brucei or Trypanosoma evansi and treated with different formulations of Berenil. Survival, parasitemia, body weight and behavior were recorded.

Results: Berenil formulated with chitosan reached a lethal concentration for bloodstream parasites. Residual parasites were absent, as demonstrated by immunosuppression.

Conclusion: The intranasal route is an effective, safer, and easier way to perform antiparasitic treatments in animals.

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Published

2021-12-03

How to Cite

1.
Fuchs AG, Soraires Santacruz MC, Fusco OA, Hoffer AM, Zhang C-X, Zhang F, Esteva MI, Rial MS, Fichera LE, Cid NG, Glisoni RJ, Lai D-H, Bontempi EJ. Trypanocidal effect of Diminazene aceturate by intranasal administration. Comparison among formulations. Int Jour HS [Internet]. 2021 Dec. 3 [cited 2022 Jan. 24];(1). Available from: https://ijhsc.com/journal/article/view/33

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